Device

Part:BBa_K4175011:Design

Designed by: Li Xianxiu, Zhang Wanying   Group: iGEM22_ZJUintl-China   (2022-09-25)


IL-6R-PD1


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1151
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 60
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1151
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 60
    Illegal BamHI site found at 76
    Illegal XhoI site found at 19
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1151
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 60
    Illegal XbaI site found at 271
    Illegal PstI site found at 55
    Illegal PstI site found at 1117
    Illegal PstI site found at 1151
    Illegal NgoMIV site found at 669
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence has been codon optimized for human.


Source

human extracellular IL-6R (BBa_K4175002), human intracellular PD-1 (BBa_K4175003)

References